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coli DH5 containing the ALR PCR product was grown on a luria-bertani broth plate containing
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onto Luria agar (minus NaCl + 6% sucrose), and select plates
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plating and on Luria broth agar containing (LB) 15gmL gentamycin and 1gmL Skin was placed on irgasan.. 96-well containing plates
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of sterile Luria Broth (LB)
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containing ampicillin (100 mgL-1), IPTG (1 mmolL-1), and X--gal (20. The total library, usually in the range of 105106 clones, is subdivided into pools of 1000 colonies, which are plated onto 9 cm Luria Broth plates. CaCl2 Cold 1 Bottle ReadyPour Luria Broth Agar, sterile 1 Bottle Luria Broth Media for recovery, sterile 40 Petri plates, small 5 Petri
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were on a Luria broth incubated kanamycin (30 gml) plate at overnight 37C. colonies 32 were picked mixed and with 10 l sterile of Luria water.. broth, L agar, and phosphate were prepared as buffer described by and AKASAKA YAMAMOTO
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37 at C. rapid For Luria broth plate. (tryptone g, 10 yeast extract 5 NaCl 10 gl g, of water) deionized was. on agar plate amended Luria with 100, and 200 150 of mgl Deborah T. Goldberg by - 2004 - Aids Study 496 pages - Fusidic acid-resistance population analyses
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agar plates. Plates were incubated at 37 C overnight... LBAMP (Luria Broth with Ampicillin) plates (3 per group - 1 for pLux, 1 for pGFP,. Record the number of colonies observed
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